Table 2.
Amplification system of real-time PCR
| QuantiTect SYBR green | 12.0 μl |
| Upstream primer (10 ng/μl) | 1.0 μl |
| Downstream primer (10 ng/μl) | 1.0 μl |
| cDNA | 4.0 μl |
| Nuclease-free H2O | 2.0 μl |
| Total volume | 20 μl |
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