Table 2.
Sequences and melting temperatures of CNAgm containing duplexes.
| Entry | Sequencea | T m b (°C) | ΔT m (°C) |
|---|---|---|---|
| 1 | 5′-GCGTTTTTTGCT-3′ 3′-CGCAAAAAACGA-5′ |
49.0 | — |
| 2 | 5′-GCGTTTxT TTGCT-3′ 3′-CGCAAAAAACGA-5′ |
55.0 | +6.0 |
| 3 | 5′-GCGTTTxTTxT GCT-3′ 3′-CGCAAAAAACGA-5′ |
59.0 | +10.0 |
| 4 | 5′-GCGTxTTxTTxT GCT-3′ 3′-CGCAAAAAACGA-5′ |
64.0 | +15.0 |
| 5 | 5′-GCAAAAACTTGC-3′ 3′-CGTTTTTGAACG-5′ |
48.0 | — |
| 6 | 5′-GCAAAAACTxT GC-3′ 3′-CGTTTTTGAACG-5′ |
53.2 | +5.2 |
| 7 | 5′-GCAAAAACTTGC-3′ 3′-CGTTTTxT GAACG-5′ |
52.2 | +4.2 |
| 8 | 5′-GCAAAAACTTGC-3′ 3′-CGTTTxT TGAACG-5′ |
54.3 | +6.3 |
| 9 | 5′-GCAAAAACTTGC-3′ 3′-CGTTxT TTGAACG-5′ |
53.4 | +5.4 |
| 10 | 5′-GCAAAAACTTGC-3′ 3′-CGTxT TTTGAACG-5′ |
52.4 | +4.4 |
| 11 | 5′-GCAAAAACTxT GC-3′ 3′-CGTTTTxT GAACG-5′ |
55.9 | +7.9 |
| 12 | 5′-GCAAAAACTxT GC-3′ 3′-CGTTTxT TGAACG-5′ |
56.4 | +8.4 |
| 13 | 5′-GCAAAAACTxT GC-3′ 3′-CGTTxT TTGAACG-5′ |
57.6 | +9.6 |
| 14 | 5′-GCAAAAACTxT GC-3′ 3′-CGTxT TTTGAACG-5′ |
57.8 | +9.8 |
a TxT : (R C5′, S P) α, β-D-CNA TT (CNAgm) within the strand. bUV melting experiments were carried out in sodium phosphate buffer (10 mM, pH 7.0) containing NaCl (100 mM) and EDTA (1 mM).