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. Author manuscript; available in PMC: 2013 Oct 2.
Published in final edited form as: Anal Chem. 2012 Sep 13;84(19):8164–8170. doi: 10.1021/ac301235a

Scheme 1. * Schematic depicting QCM assay used to detect heat-denatured Herceptin.

Scheme 1

(i) NeutrAvidin was drop-coated onto the freshly prepared bare Au quartz crystal electrode; (ii) Biotinylated 2B4 scFv were tethered to the Au surface electrode via biotin-NeutrAvidin interaction; (iii) Heat-denatured Herceptin was captured by the 2B4 scFv on the Au quartz crystal electrode and detected by the QCM assay (No response to the intact Herceptin was observed). * Note: Figure not drawn to scale.