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. 2012 Aug 1;5:12. doi: 10.1186/1755-1536-5-12

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Copyright ©2012 Iizuka et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of miR-214 overexpression on mRNA expression in LX-2 cells. (A) LX-2 cells were transfected with a miR-214 precursor or a negative control (control) at a final concentration of 50 nM and incubated for 24 hours. miR-214 expression was quantitated using real-time PCR. (B) The expression of fibrosis-related genes in LX-2 cells transfected with miR-214 precursors was analyzed using real-time PCR. The results are expressed as the expression relative to that in cells transfected with the control. **P < 0.01.