Table 1.
Nucleotide primer sequences used for real-time quantitative PCR
| Target genes | Nucleotide sequence | Orientation | Amplicon length | Accession No. |
|---|---|---|---|---|
| Bak |
5′— CCCAGGACACAGAGGAGGTTT—3′ 5′— GCCTCCTGTTCCTGCTGATG—3′ |
Sense Antisense |
65 bp |
NM001188 |
| Bcl-xl |
5′— TGCGTGGAAAGCGTAGACAA—3′ 5′—ATTCAGGTAAGTGGCCATCCAA—3′ |
Sense Antisense |
75 bp |
NM138578 |
| Mcl-1 |
5′— AGGCTGGGATGGGTTTGTG—3′ 5′—CACATTCCTGATGCCACCTTCT—3′ |
Sense Antisense |
65 bp |
NM021960 |
| GAPDH | 5′- AACAGCCTCAAGATCATCAGCA-3′ 5′- CATGAGTCCTTCCACGATACCA-3′ |
Sense Antisense |
102 bp | M33197 |