Table 2.

Nucleotide sequence of PCR primers used for the construction of FMDV O/HN/CHA/93 full-length cDNA clone

Name	Nucleotide Sequence (5′ → 3′)	Nucleotide Position (nt)
Z1	AGACTAGTtaatacgactcactataT7promoterGGGTTG	1–20
	AAAGGGGGCGCTAGGGT
Z1′	GAGGAGGGGGGGGGGGGGGGTGAAAG	363–388
Z2	CTTTCACCCCCCCCCCCCCCCTCCTC	363–388
Z2′	GAAGCAACAGTGCTGCTACT	721–740
Z3	CGCGCCGTCGCTTGAGGAAG	560–579
Z3′	GGGTCGGAGCTCCTCCTTGATAGA	5433–5456
Z4	AGCCACCTCTTCAGAACGTCTACC	5242–5265
Z4′	ACGCGGCCGCTTTTTTTTTTTTTTTTTTTTT	3′ end
Ztu1	GGAGAGCGAGTCAGATATCTTCTTCACTACA	4221–4251
Ztu1′	TGTAGTGAAGAAGATATCTGACTCGCTCTCC	4221–4251
Ztu2	GAAAACGCCTGAGGCCGCCGCACACTGCATT	2967–2997
Ztu2′	AATGCAGTGTGCGGCGGCCTCAGGCGTTTTC	2967–2997

Restriction enzyme sites introduced during clone construction are boxed. Mutated nucleotides are underlined and in bold.