Figure 4 .

Matrix metalloproteinases inhibitors modulate the proliferative response induced by sPLA₂-IIA in BV-2 cells. BV-2 cells were treated for 30 minutes at 37°C in the presence or absence of the indicated inhibitors, and then were stimulated with 1 μg/ml of sPLA₂-IIA. (A) After 24 h, cell proliferation was investigated and expressed as optical density. values ± SD. Values are the average of three separate experiments in quadruplicate (*P < 0.001 compared to control cells, **P < 0.001 compared to sPLA₂-IIA-treated cells). (B) After 15 minutes, ERK 1/2, P70S6 and rS6 phosphorylation were identified in the cell lysates by western blot. Membranes were stained with Ponceau S as a loading control. (C) Phosphorylation levels of EGFR on untreated (open black curves), 1 μg/ml of sPLA₂-IIA-treated (open dark grey curves), and MMP inhibitors + 1 μg/ml of sPLA₂-IIA-treated (open blue curves) BV-2 cells were analyzed by flow cytometry and compared with isotype controls (solid grey curves). Results shown are representative of three independent experiments.