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. 2012 Aug 11;9:195. doi: 10.1186/1742-2094-9-195

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Copyright ©2012 Vartak-Sharma and Ghorpade; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Astrocyte elevated gene-1 (AEG-1) colocalizes with reactive astrocytes during astrogliosis. AEG-1 expression in activated astrocytes of mouse brain, 4 days post stereotactic phosphate-buffered saline (PBS) injection, was analyzed by immunofluorescent microscopy. The non-injected contralateral hemisphere was used as a control. Immunostaining for glial fibrillary acidic protein (GFAP) (red, astrocyte marker) and 4', 6-diamidino-2-phenylindole (DAPI) (blue, nuclear marker) identified the needle tract of activated astrocytes (A,B). Non-injected (inset image, (C) and PBS-injected contralateral hemispheres costained for AEG-1 and GFAP, were assessed for AEG-1 expression and colocalization with GFAP (yellow, inset image, (D).