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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Breast Cancer Res Treat. 2012 Sep 2;136(1):21–34. doi: 10.1007/s10549-012-2224-0

Fig. 5.

Fig. 5

Effects of Pg on AKT-associated kinase pathways in BT474 and MDA-MB-231 cells. (a) Effects of Pg on SHIP-1 mRNA levels and (b) SHIP-1, pP13K and pAKT protein expression in BT474 cells. Cells were treated with solvent DMSO (control) or different concentration of Pg (2.5–10 µg/ml) for 24 h (c) SHIP-1 mRNA levels and protein expression of SHIP-1, pP13K and pAKT in MDA-MB-231 cells. Cells were treated with solvent DMSO (control) or different concentration of Pg (2.5–10 µg/ml) for 24 h (d) Effect of SiRNA SHIP-1 with and without Pg on pAKT protein (e) Basal levels of miR-155 in different breast cancer and non-cancer breast fibroblasts. (f) Effects of Pg on miR-155 levels in different breast cancer and non-cancer breast fibroblasts. (g) Effects of Pg with and without Ant. miR155 in BT474 cells on the expression of miR155 and (H) SHIP-1 mRNA levels. All experiments were performed as described in the material and method section and were performed at least three times, and result were expressed as mean ± SE.* indicates significant changes at p<0.05.