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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Jun 20;424(1):58–64. doi: 10.1016/j.bbrc.2012.06.065

Fig. 1.

Fig. 1

TNFα enhanced CSC phenotype in OSCC. Three OSCC cell lines (SCC4, SCC9, and HEp2) were exposed to 5–10ng/ml of TNFα for 2 months. (A) Tumor sphere formation assay was performed in triplicate. P values were obtained against untreated corresponding control. (B) Representative image of tumor spheres formed by SCC9 and TNFα-exposed SCC9 (SCC9/TNF). The photographs were taken at a magnification of 40X. (C) qPCR analysis of stemness- associated genes in SCC9 and SCC9/TNF. Levels of the stemness-associated genes in SCC9/TNF were plotted as fold induction against those in SCC9. (D) Chemo-radioresensitivity assay. Cells were treated with 40μM of etoposide for 2, 4, and 6 days, and their viability was measured using MTT assay (left). For clonogenic survival assay (right), cells were irradiated with 5 and 10 Gy, and surviving colonies were stained and counted (right). Data are expressed as the mean ± SD of triplcate.