Skip to main content
. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Jun 20;424(1):58–64. doi: 10.1016/j.bbrc.2012.06.065

Fig. 3.

Fig. 3

Role of Notch1 signaling pathway in CSC property in the TNFα-exposed SCC9 cells. (A) Western blot analysis of NICD and its target Hes1 in SCC9 and SCC9/TNF. GAPDH was used as a loading control. (B) qPCR analysis of NICD target genes in SCC9 and SCC9/TNF. Levels of the genes in SCC9/TNF were plotted as fold induction against those in SCC9. (C) Synthetic γ-secretase inhibitor, DAPT (5μM) abrogated the expression of NICD and Hes1 protein in SCC9/TNF as determined by Western blot analysis. The cells treated with DMSO were included for comparison. (D) Inhibition of Notch 1 pathway reduced tumor sphere-forming ability of SCC9/TNF. The SCC9/TNF cells were treated with DAPT for 2 days and subjected to tumor sphere-forming assay in triplicate. P value was obtained against DMSO-treated control.