Figure 3.

KAPP and CLV1 associated in vivo. Antibodies raised to recombinant KAPP and CLV1 were used to co-immunoprecipitate the proteins from cauliflower meristem extracts. A and B, Demonstration of the specificity of CLV1 antibodies. Extracts (25 μg lane⁻¹) from mutant (clv1-6) and wild-type (Landsberg erecta [Ler]) Arabidopsis inflorescence tissue, including mature flowers, cauline leaves, and some stem tissue, were resolved by SDS-PAGE. A, Coomassie blue-stained gel. B, Results of chemiluminescence detection of immunoreactive polypeptides after incubation with CLV1 antisera. C, Proteins extracted from cauliflower (Caul.) and Arabidopsis (Arab.) were subjected to immunodetection using affinity-purified KAPP antibodies. D and E, Cauliflower meristematic tissue extracts were incubated with immobilized preimmune (PI) or immune (I) antisera against CLV1. Immunocomplexes were eluted, resolved by SDS-PAGE, and immunodetected with immune antisera against CLV1 (D) or KAPP (E). The positions of molecular mass standards are indicated on the left of each panel in kilodaltons.