Skip to main content
. 2012 Oct;47(4):528–535. doi: 10.1165/rcmb.2012-0127OC

Figure 6.

Figure 6.

WISP1 interacted with Toll-like receptor (TLR) 4 in VILI. A/J mouse lung tissues obtained from 4-hour CON or HTV mechanical ventilation were homogenized and total lysates were immunoprecipitated (IP) with anti-WISP1 antibody or anti–TLR4–MD2 antibody before immunoblot (IB) was performed. (A) Glycosylated form of TLR4 (120 kD) was detected with anti-TLR4 antibody (IB: TLR4) either in the whole lung tissue lysates (Total Lysates) or after immunoprecipitation with anti-WISP1 antibody (IP: WISP1). (B) WISP1 was detected with anti-WISP1 antibody (IB: WISP1) either in the whole lung tissue lysates (Total Lysates) or after immunoprecipitation with anti–TLR4–MD2 antibody (IP: TLR4-MD2). Antibody species-matched serum (Rat IgG) was used as a negative control for coprecipitation experiments. Results are representative of tests performed on three occasions.