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. 2012 Oct;47(4):445–453. doi: 10.1165/rcmb.2011-0332OC

Figure 2.

Figure 2.

(A) PLY (30 ng/ml) increases RhoA GTPase activity (assessed with a commercially available G-LISA kit; Cytoskeleton, Denver, CO) within 2 hours in HL-MVECs (n = 8). TIP peptide (27 μM), when applied 30 minutes before PLY, significantly blunts this effect of PLY. (B) Two-hour PLY (30 ng/ml) treatment of HL-MVECs significantly reduces Rac1 activity (n = 8; assessed with a commercially available G-LISA kit), an effect blocked upon a 30-minute pretreatment with TIP peptide (27 μM). (C) PLY (30 ng/ml) increases myosin light chain (MLC) phosphorylation within 2 hours in HL-MVECs as assessed by Western blotting (n = 3) and quantified as (D) the densitometric units ratio of ppMLC over total MLC normalized to controls (n = 6).