Figure 4.

CDC reduces LPS-induced lung inflammation. ALI was induced by an intratracheal administration of LPS (50 μg). CDC (30 μg) or vehicle (CD) was administered 2 hours later. Bronchoalveolar lavage fluid (BALF) was collected, and lungs were excised after 4 hours. (A) BALF was centrifuged at 500 × g for 5 minutes. Pelleted cells were washed, and the cellular content of the resuspended pellet was determined via cytospin. (B) Cells from BALF were stained with Diff-Quik and examined microscopically. (C) Nuclear protein was isolated from lungs, and NF-κB activity was determined using an ELISA-based assay kit (top left). Lung tissue was homogenized, and oxidant stress was assessed by measuring H₂O₂ production (top right), nitrate (bottom left), and malonaldehyde (MDA; bottom right), using commercially available kits (n = 16–18 mice/group). ***P < 0.001. n.s., not significant; PMNs, polymorphonuclear leukocytes; Veh, vehicle.