Figure 5.

CDC attenuates lung injury and edema after LPS administration. ALI was induced by intratracheal administration of LPS (50 μg). CDC (30 μg) or vehicle (CD) was administered 2 hours later, and BALF was collected after 4 hours. (A) Left: Cells in BALF were pelleted by centrifugation, after which the protein content of the supernatant was determined as a marker of lung injury. Before BALF was collected, the superior lobe of the left lung was excised and immediately weighed. It was then dried in an incubator at 60°C for 24 hours and weighed again. Right: The ratio of wet/dry weight was determined as an index of edema. (B) Top: Lungs were fixed, sectioned, and stained with hematoxylin and eosin. Representative sections were then examined microscopically. Bottom: The degree of injury (Pathology Scores) was calculated according to the scale: 1, no injury; 2, injury to 25% of the field; 3, injury to 50% of the field; 4, injury to 75% of the field; and 5, diffuse injury (n = 16–18 mice/group). **P < 0.01. ***P < 0.001.