Figure 4.

Summary results and representative examples of Western immunoblots probed for talin (top), α-actinin (middle), and vinculin (below) in RV myocardium obtained at the end of RVPO in pigs treated with vehicle (V) or the calpain inhibitor MDL-28170 (M). Band intensities of talin, α-actinin, vinculin, and α-tubulin were each normalized to the mean of a fiduciary set of four samples that were repeated on each gel; α-tubulin was used as a loading standard. The talin/α-tubulin ratio was 21% higher in pigs treated with MDL-28170 than in pigs treated with vehicle (P = 0.039). A trend toward a higher α-actinin/α-tubulin ratio (9%) in MDL-28170 pigs was evident, but this difference did not reach statistical significance (P = 0.12). No difference in vinculin/α-tubulin ratio was evident between the two groups.