Figure 2.

CCH restored high-affinity iron uptake to atx1 mutant yeast. The atx1 mutant strain SL215 was transformed with vector p413-A1 (ATX1 expression), pRS413 (expression vector, negative control), pB-038 (CCH expression), or pSM703 (expression vector, negative control) and streaked on plates in the positions indicated. All plates contained ferrozine, an iron chelator. Plates contained synthetic dextrose medium plus 3 mm ferrozine (SD+fer) or synthetic dextrose medium plus 3 mm ferrozine plus 50 μm ferrous ammonium sulfate (SD+fer+Fe). Plates were photographed after incubation at 30°C for 4 d.