CCH restored high-affinity iron uptake to
atx1 mutant yeast. The atx1 mutant strain
SL215 was transformed with vector p413-A1 (ATX1
expression), pRS413 (expression vector, negative control), pB-038
(CCH expression), or pSM703 (expression vector, negative
control) and streaked on plates in the positions indicated. All plates
contained ferrozine, an iron chelator. Plates contained synthetic
dextrose medium plus 3 mm ferrozine (SD+fer) or synthetic
dextrose medium plus 3 mm ferrozine plus 50
μm ferrous ammonium sulfate (SD+fer+Fe). Plates were
photographed after incubation at 30°C for 4 d.