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CCH can restore Lys and Met synthesis in aerobically grown sod1 mutant yeast. Strain KS107 (sod1) was plated after transformation with no expression vector (A, negative control), pRS-A1 (B, ATX1 expression), or pB-038 (C, CCH expression). Plates contained complete medium (SD) or medium lacking Met (−met) or Lys (−lys). Plates were photographed after aerobic growth at 30°C for 2 d.
