Figure 4.

CCH rescued sod1 mutant yeast in a copper-dependent manner. A, Strain KS107 (sod1Δ) was transformed with the vector pSM703 (+vector) as a negative control or pB-038 (+CCH) as the CCH expression vector. Yeast was grown in yeast extract-peptone-dextrose medium alone or yeast extract-peptone-dextrose plus 150 μm BCS with or without 50 μm CuSO₄. A₆₀₀ was determined after yeast was grown in either aerobic or anaerobic conditions for 18 h beginning at A₆₀₀ = 0.01. B, A, SL201 (sod1Δ,ctr1Δ) plus pSM703 (empty expression vector); B, KS107 (sod1Δ) plus pSM703; C, SL201 plus pB-038 (CCH expression); and D, KS107 plus pB-038 yeast were grown in aerobic or anaerobic conditions for 2 d on synthetic dextrose medium lacking Lys.