Skip to main content
. 2004 Feb;70(2):1199–1206. doi: 10.1128/AEM.70.2.1199-1206.2004

FIG. 1.

FIG. 1.

(A) Organization of Alexandrium species rDNA and location of primers for the Alexandrium genus-specific PCR. (B) Amplification of target DNA in real-time PCR followed by analysis on standard agarose gel. Lanes: 1, template DNA from A. catenella CSIC-4; 2, template DNA from A. taylori CBA-1; 3, template DNA from A. minutum CNR-AMIA4; 4, template plasmid containing A. catenella ITS1-5.8S-ITS2 rDNA; 5, template plasmid containing A. taylori ITS1-5.8S-ITS2 rDNA; M, 100-bp ladder molecular size marker. A clean no-template control was included in the real-time assay (not shown on the gel). NTS, nontranscribed spacer; SSU, small subunit; LSU, large subunit.