TABLE 2.
Specificity of real-time PCR
| Species | Strain | Phylogenetic association | Amplificatione |
|---|---|---|---|
| Pseudonitzschia pseudodelicatissima | IEOa-PS 50V | Diatom (Bacillariaceae) | − |
| Gyrodinium corsicum | GC1 CSICb-1 | Dinoflagellate (Gymnodiniaceae) | − |
| Karlodinium micrum | KM1 CSICb-1 | Dinoflagellate (Gymnodiniaceae) | − |
| Coolia monotis | IEOa-CM7C | Dinoflagellate (Ostreopsidaceae) | − |
| Ostreopsis sp. | IEOa-OSBR3 | Dinoflagellate (Ostreopsidaceae) | − |
| Dinophysis sacculus (ITS1-5.8S-ITS2 cloned sequence) | No strain | Dinoflagellate (Dinophysidae) | − |
| Alexandrium minutum | CNRc-AMIA4 | Dinoflagellate (Gonyaulacaceae) | + |
| Alexandrium catenella | CSICb-4 | Dinoflagellate (Gonyaulacaceae) | + |
| Alexandrium taylori | CBAd-1 | Dinoflagellate (Gonyaulacaceae) | + |
a
Instituto Español de Oceanografia, Vigo, Spain.
b
Institut de Ciències del Mar, Barcelona, Spain.
c
Consiglio Nazionale delle Ricerche, Messina, Italy.
d
Centro Biologia Ambientale, Urbino University, Italy.
e
−, amplification products not detected; +, amplification products detected.