Table 1. Expression of proteins that regulate lipolysis or lipogenesis: comparison of VGF knockout and wild type gonadal WAT.

Protein levels were determined in gonadal fat pads from 9–10 week old male and female mice using western blot analysis as described in Materials and Methods.

Protein Quantified in Male WAT	Protein Expression (Arbitrary Units) Mean ± SEM	P- Value	Protein Quantified in Female WAT	Protein Expression (Arbitrary Units) Mean ± SEM	P- Value
FAS	Vgf+/+ = .19±.21 Vgf−/− = .17±.06	.93	FAS	Vgf+/+ = .27±.04 Vgf−/− = .37±.11	.45
Perilipin	Vgf+/+ = .46±.12 Vgf−/− = .55±.05	.62	Perilipin	Vgf+/+ = 1.14±.22 Vgf−/− = 1.01±.32	.79
TORC3	Vgf+/+ = .015±.003 Vgf−/− = .017±.002	.55	TORC3	Vgf+/+ = .03±.01 Vgf−/− = .04±.01	.58
pCREB	Vgf+/+ = .006±.002 Vgf−/− = .002±.001	.25	pCREB	Vgf+/+ = .01±0.00 Vgf−/− = .01±0.00	.30
IRbeta	Vgf+/+ = .06±.003 Vgf−/− = .08±.02	.55	IRbeta	Vgf+/+ = .05±0.01 Vgf−/− = .07±0.02	.57
ACC	Vgf+/+ = .008±.002 Vgf−/− = .074±.046	.23	ACC	Vgf+/+ = .114±.06 Vgf−/− = .077±.02	.63
pACC	Vgf+/+ = .15±.061 Vgf−/− = .42±.146	.16	pACC	Vgf+/+ = .39±.104 Vgf−/− = .22±.045	.19
p42/44 MAPK	Vgf+/+ = .41±.053 Vgf−/− = .56±.044	.11	p42/44 MAPK	Vgf+/+ = .42±.04 Vgf−/− = .41±.015	.99
phospho- p42/44 MAPK	Vgf+/+ = .04±.002 Vgf−/− = .25±.083	.06	phospho- p42/44 MAPK	Vgf+/+ = .06±.008 Vgf−/− = .17±.065	.18
Glut4	Vgf+/+ = .03±.006 Vgf−/− = .04±.021	.58	Glut4	Vgf+/+ = .038±.009 Vgf−/− = .057±.008	.20
HSL total	Vgf+/+ = .16±.017 Vgf−/− = .14±.060	.80	HSL total	Vgf+/+ = .15±.061 Vgf−/− = .076±.024	.32

All data are normalized to the loading control β-actin, and are expressed in arbitrary units (mean ± SEM); statistical significance was determined using the two-tailed Student’s t test with *p < 0.05 considered significant [male, n=3–6 (Vgf+/Vgf+), n=3–5 (Vgf−/Vgf−); female, n=3–4 (Vgf+/Vgf+), n=3–5 (Vgf−/Vgf−)].

No significant differences in the levels of these proteins were found between Vgf−/Vgf− and Vgf+/Vgf+ WAT.