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. Author manuscript; available in PMC: 2012 Nov 5.
Published in final edited form as: Cell. 2012 Jan 20;148(1-2):296–308. doi: 10.1016/j.cell.2011.11.057

Figure 4. Mutant Rnu2–8 Acts in a Dosage-dependent Fashion on Neuron Survival.

Figure 4

(A–F) Hematoxylin and eosin stained sagittal sections of NMF291+/− and wild type (+/+) cerebella. (E, F) Higher magnification images of lobule IV/V from (C) and (D).

(G) The 1.5kb DNA fragment, containing the transcription unit of the mutant Rnu2–8 and its 5' and 3' flanking sequences, used for pronuclear injection.

(H) RPA analysis of cerebellar RNA from P30 wild type, NMF291+/−, NMF291−/− mice and mice hemizygous for the mutant Rnu2–8 transgene (Tg).

(I) RPA analysis of cerebellar RNA from P20, P30, and P40 NMF291−/− mice and mice hemizygous for the transgene (Tg).

(J) RPA analysis of tissues from P30 NMF291−/−and hemizygous Tg-MuU2 mice. Note the NMF291−/− cerebellar lane was run on a separate gel.

(K and L) Hematoxylin and eosin stained sagittal sections of Tg-MuU2 cerebella. DES, distal sequence element; PES, proximal sequence element. Scale bars: (D and L) 500 μm; (F) 50 μm. See also Figure S4.