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. Author manuscript; available in PMC: 2012 Nov 5.
Published in final edited form as: Brain Res. 2008 Jul 29;1233:63–78. doi: 10.1016/j.brainres.2008.07.076

Figure 6.

Figure 6

MTS assay showing influence of TAT-HA-PRDX6 in protecting RGC-5 against glutamate (A)- or TNF-α (B)- or their synergistically-induced cytotoxicity (C). Cells were seeded (10K cells/well) in 24 well plates containing complete DMEM. An overnight culture of these cells were subjected to varying concentrations of TAT linked PRDX6. Three hours later cells were exposed to glutamate (A) or TNF-α (B) or glutamate plus TNF-α. After 24hr, cell viability was estimated using MTS assay. Protective ability of TAT-HA-PRDX6 is evident (white bars). Results are presented in percent survival. While TAT-HA-PRDX6 failed to attenuate glutamate, TNF-α and/or their synergistically-induced cytotoxic activity against RGC-5 (Fig. 6, A: gray bar; B: stripped bar; C: dotted bar. Results are means ± SD of three individual experiments. **; p< 0.001