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Depletion of contactin2 by RNAi. HEK cells were cotransfected with two plasmids, one encoding Cntn2 and the other encoding GFP (a, a′, a″) or GFP and microRNA Cntn2C (b, b′, b″). The microRNA dramatically decreased Cntn2 levels. Note that most of the Cntn2-positive cells in b do not coexpress microRNA, likely due to imperfect cotransfection. Scale bar, 10 μm.
