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. 2012 Sep 11;120(18):3783–3792. doi: 10.1182/blood-2012-04-424630

Figure 4.

Figure 4

Autocrine or paracrine gp80 protects MCL cells against apoptosis. Percentages of apoptotic: (A) Mino cells in cultures with or without addition of IL-6 (0.5 ng/mL), gp80-neutralizing antibodies (α-gp80; 10 μg/mL), or control IgG1 for 2 hours before BTZ (10nM) was added to the culture for additional 48 hours; (B) gp80-knockdown (KD), wild-type (WT), or empty vector-transfected Mino cells (Empty vector) in cultures with or without addition of IL-6 (0.5 ng/mL) or Minosup for 2 hours before BTZ (10nM) was added to the culture for additional 48 hours; or (C) SP53 cells in cultures with or without addition of IL-6 (0.5 ng/mL), Minosup, gp80-neutralizing antibodies (α-gp80; 10 μg/mL) or control IgG1 for 2 hours before BTZ (10nM) was added to the culture for additional 48 hours. (D) ELISA showing the levels of gp80 secreted by conditional, gp80-expressing stable SP53 cells induced by doxycycline (DOX) or vehicle. (E) Percentage of apoptotic gp80-transfected (S80), empty vector-transfected (Svec), or parental SP53 cells, pretreated with PBS or DOX overnight, in cultures with addition of gp80-neutralizing antibodies (α-gp80; 10 μg/mL) or control IgG1, and/or BTZ (10nM) for 48 hours. Results of 3 independent experiments are shown. The P values in the graphs show comparison as indicated.