FIG. 3.

Formation of NDAB in aerobic soil slurries (20%, wt/vol) prepared with an ammunition plant soil contaminated with RDX (342 μmol kg⁻¹), HMX (3,057 μmol kg⁻¹), MNX (155 μmol kg⁻¹), and traces of NDAB (3.8 μmol kg⁻¹) in m-succinate medium inoculated with either RDX-induced Rhodococcus sp. strain DN22 (▪), Rhodococcus sp. strain A (○), or R. rhodochrous sp. strain 11Y (▵). The strains were induced with RDX, harvested at mid-log phase and concentrated to an optical density at 530 nm of approximately 4.0. The bacterial suspensions (0.6 ml) were used to inoculate the slurries. Incubation was performed at 30°C with agitation at 180 rpm. Values represent the averages and standard deviations of triplicate experiments.