Table 3.
The weight and character of the seven different active fractions following silica gel quick column chromatography of the DCM extract of bee corn pollen (CDEb)
| Fraction | Weight (mg) |
Yield
a
(CDEb) |
Yield
b
(Bee pollen) |
Character | Activity c |
|---|---|---|---|---|---|
| CDEb1 |
62 |
2.58 |
0.01 |
Clear white solid |
-- |
| CDEb2 |
42 |
1.75 |
0.01 |
Pale yellow solid |
-- |
| CDEb3 |
6 |
0.25 |
0.00 |
Pale brown solid |
-- |
| CDEb4 |
5 |
0.21 |
0.00 |
Pale brown solid |
+ |
| CDEb5 |
776 |
32.3 |
0.18 |
Dark brown solid |
+++ |
| CDEb6 |
430 |
17.9 |
0.10 |
Sticky, dark brown solid |
+++ |
| CDEb7 | 760 | 31.7 | 0.18 | Dark brown solid | ++ |
a,bYield as a % of the aCDEb fraction and bof the initial bee pollen mass
cQualitative free radical scavenging activity, as determined by the DPPH assay sprayed onto the TLC plates, where -, +, ++ and +++ represent no, weak, moderate and strong color change (activity).