Table 4.
The percentage scavenging activity of the crude DCM extract of bee pollen (CDEb) and its subfractions (CDEb1 - CDEb7) following silica gel 60 quick column chromatography
| Fraction | Percentage scavenging activity | EC 50 | r 2 | ||||
|---|---|---|---|---|---|---|---|
| |
10 μg/ml |
50 μg/ml |
100 μg/ml |
400 μg/ml |
1000 μg/ml |
(μg/ml) |
|
| CDEb1 |
3.76 ± 4.27 |
0.04 ± 2.05 |
0.31 ± 2.54 |
0.00 |
8.29 ± 2.41 |
>1000 |
|
| CDEb2 |
3.16 ± 1.47 |
0.00 |
0.00 |
0.00 |
5.92 ± 1.85 |
>1000 |
|
| CDEb3 |
1.43 ± 2.07 |
0.1 ± 1.58 |
0.00 |
0.00 |
9.05 ± 0.54 |
>1000 |
|
| CDEb4 |
0.00 |
0.00 |
0.00 |
14.35 ± 5.91 |
12.0 ± 0.54 |
>1000 |
|
| CDEb5 |
5.15 ± 0.7 |
14.0 ± 3.04 |
22.5 ± 1.86 |
59.8 ± 3.98 |
65.3 ± 0.8 |
322 ± 27 |
1 |
| CDEb6 |
3.69 ± 3.7 |
19.4 ± 3.80 |
37.6 ± 1.33 |
76.7 ± 1.2 |
78.4 ± 1.44 |
196 ± 9.9 |
1 |
| CDEb7 |
3.34 ± 1.33 |
6.19 ± 2.13 |
9.69 ± 0.8 |
38.3 ± 4.8 |
58.5 ± 1.71 |
762 ± 67 |
1 |
| CDEb |
66.9 ± 0.88 |
68.8 ± 0.47 |
70.8 ± 0.73 |
70.1 ± 0.47 |
61.8 ± 0.7 |
7.47 ± 0.12 |
1 |
| Ascorbic acid | 58.4 ± 1.7 | 76.9 ± 1.3 | 76.8 ± 1.05 | 76.1 ± 0.72 | 65.2 ± 6.5 | 8.57 ± 0.24 | 1 |
The reference standard, ascorbic acid, is shown for comparison.
EC50 values were calculated by linear regression (r2 represents the correlation coefficient). ND represents no available data. Data are shown as the means ± 1 SD and are derived from 3 independent repeats.