Table 5.
The weight and character of the pooled active fractions derived from the Sephadex LH-20 size exclusion chromatography of the bee pollen derived CDEb-5 fraction
| Fraction | Weight (mg) |
Yield
a
(CDEb-5) |
Yield
b
(Bee pollen) |
Character | Activity c |
|---|---|---|---|---|---|
| CDEb5-1 |
28.1 |
3.62 |
0.01 |
Brown solid |
|
| CDEb5-2 |
188.4 |
24.3 |
0.04 |
Dark brown solid |
|
| CDEb5-3 |
7.8 |
1.01 |
0.00 |
Dark brown solid |
|
| CDEb5-4 |
3.9 |
0.50 |
0.00 |
Brown solid |
|
| CDEb5-5 |
2.8 |
0.36 |
0.00 |
Brown solid |
++ |
| CDEb5-6 | 9.9 | 1.28 | 0.00 | Yellow-green solid | ++ |
a,bYield as a % of the aCDEb5 fraction and bof the bee pollen
cQualitative free radical scavenging activity, as determined by the DPPH assay sprayed onto the TLC plates, where -, +, ++ and +++ represent no, weak, moderate and strong color change (activity).