Table 1.
List of primers with annealing temperature and product size (bp)
| Exon No | Primer sequence (Forward) F | Primer sequence (Reverse) R | Product size (bp) | Annealing temperature °C |
|---|---|---|---|---|
| 1 |
TCACGTGCCCAGAACGTC |
CTCATTCCTGGGAAGGGACT |
266 |
56 |
| 2 |
AAGCGACTTTTGGTGATAGGA |
TTACTCCCCACGCCCAAC |
308 |
54 |
| 3 |
GAGAGGCCATGTTGGGTTAA |
TCCACCTCTCAATGCCTACC |
222 |
58 |
| 4 |
TCTGGGATTTCAGGTATGGTG |
ATTGCTACGGGCAATCACTC |
295 |
58 |
| 5 |
GTGTTTCATGGTAACCCATGG |
TTTATGAACAAGCGATTTGGG |
219 |
58 |
| 6 |
TCTTTGAAAATGACTGCTACCTT |
CCTGGGTTCAGCAGAAAGAG |
156 |
58 |
| 7 |
AGGACCCTCCTGACCAGAGT |
CTTTCCCTGTGCCCACAG |
205 |
60 |
| 8 | TCATGGTTTTCTGACCTTTGC | GCCCTCTCAGTGTCCAGAAG | 190 | 58 |