Figure 5.

Protoplast shrinking induced by continuous blue light. Protoplasts from the wild type (A and F) and mutants (B and G, hy4; C and H, phyA; D and I, phyB; and E and J, phyAphyB) of Arabidopsis, isolated and incubated under red light as described for Figure 1, were subjected to time-lapse photography (3-min intervals). Protoplasts were exposed to blue light (70 μmol m⁻² s⁻¹) continuously from time 0, which was 65 min after the onset of incubation. The volume of small protoplasts (left panels) and large protoplasts (right panels) was analyzed as described for Figure 2. The means ± se from 18 to 29 protoplasts are shown. Dotted lines reproduce the regression lines of Figure 2.