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. Author manuscript; available in PMC: 2013 Nov 23.
Published in final edited form as: J Chromatogr A. 2012 Oct 8;1265:114–122. doi: 10.1016/j.chroma.2012.09.091

Figure 2.

Figure 2

Example of frontal analysis studies for glibenclamide on a normal HSA column at pH 7.4 and 37°C. These results were obtained at 0.5 mL/min and using glibenclamide concentrations of 50, 20, 10, and 5 μM (top-to-bottom). The small initial step changes shown to the left occurred at or near the column void time and probably represent only a small difference in composition and background absorbance of each drug solution versus the application buffer; these small step changes were not included in the data analysis and integration of the much larger frontal analysis curves that are shown to the right.