Table 1.
Association equilibrium constants and moles of binding sites estimated when using a two-site model to describe the binding of glibenclamide with normal HSA and gHSA3 at pH 7.4 and 37 °Ca
| Type of HSAb | Ka1 (× 106 M−1) | mL1 (× 10−8 mol) | Ka2 (× 104 M−1) | mL2 (× 10−8 mol) |
|---|---|---|---|---|
| Normal HSA | 1.4 (± 0.5) | 1.1 (± 0.2) | 4.4 (± 1.0) | 3.1 (± 0.1) |
| gHSA3 | 1.9 (± 1.5) | 0.9 (± 0.3) | 7.2 (± 2.8) | 2.4 (± 0.2) |
a
The values in parentheses represent a range of ±1 S.D., as based on error propagation and the precisions of the best-fit slopes and intercepts obtained when using Eq. (3) for n = 16.
b
The level of glycation for the gHSA3 sample is given in Section 2.3.