(A - F) Photomicrographs of BrdU (green or yellow)+ cells in the DG of PS1hWT (A, D), PS1ΔE9 (B, E) and PS1M146L (C, F). SH, standard housing conditions (A, B, C); EE, enriched conditions (D, E, F). NeuN, red. Scale bar: 100 μm.
(G) Quantification of BrdU+ cells (mean ± SEM; N = 10 - 15 per group). * P < 0.05.
(H) Quantification of granule cell number. * P < 0.05.
(I) Photomicrograph of primary neurospheres. Scale bar: 50 μm.
(J) Image of nestin (green)+ neurosphere. Scale bar: 25 μm.
(K) Nuclei were stained with DAPI (blue).
(L) Percentage of multipotent neurospheres obtained in clonogenic neurosphere assay (mean ± SEM, N = 5 animals per group). ** P < 0.01.