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. 2004 Feb;70(2):1135–1144. doi: 10.1128/AEM.70.2.1135-1144.2004

FIG. 3.

FIG. 3.

Alignment of the amino acid sequences deduced from the amplified fragments of family 19 chitinases from Actinobacteria strains determined in this study and those reported previously. Residues conserved in all sequences are indicated by white type on a black background, while residues conserved in >80 and >60% of the proteins examined are indicated by white type on a dark gray background and by black type on a light gray background, respectively. Conserved regions of family 19 chitinases are indicated by the solid bars (C1, C2, C3, and C4). Catalytic amino acid residues of family 19 chitinases are indicated by arrows. 1, Glycomyces harbinensis; 2, Cellulomonas cellulans YCWD3; 3, Promicromonospora sukumoe; 4, Actinoplanes brasiliensis fragment 1; 5, Actinoplanes brasiliensis fragment 2; 6, Catenuloplanes japonicus; 7, Actinokineospora riparia; 8, Amycolatopsis orientalis subsp. orientalis; 9, Kibdelosporangium aridum subsp. aridum fragment 1; 10, Kibdelosporangium aridum subsp. aridum fragment 2; 11, Streptoalloteichus hindustanus; 12, Kitasatospora setae fragment 1; 13, Kitasatospora setae fragment 2; 14, Streptomyces coelicolor A3 (2) chiF (DNA accession no. AB017012); 15, Streptomyces coelicolor A3(2) chiG (AB017013); 16, Streptomyces coelescens ISP5421 fragment 1 (AB031749); 17, Streptomyces coelescens ISP5421 fragment 2 (AB031750); 18, Streptomyces griseus HUT6037 chiC (AB009289); 19, Streptomyces ipomoeae MAFF4023 (AB031751); 20, Streptomyces lavendulae (AF127374); 21, Streptomyces lividans 66 fragment 1 (AB031746); 22, Streptomyces lividans 66 fragment 2 (AB031747); 23, Streptomyces lividans 66 fragment 3 (AB031748); 24, Streptomyces olivaceoviridis chi30 (AJ133186); 25, Streptomyces prasinopilosus fragment 1 (AB031752); 26, Streptomyces prasinopilosus fragment 2 (AB031753); 27, Streptomyces sp. strain AJ9463 chiIS (AB104621); 28, Streptomyces sp. strain S15 (AB031754); 29, Streptomyces sp. strain S84 (AB031755); 30, Streptomyces sp. strain S100 (AB031756); 31, Streptomyces sp. strain S159 (AB031757); 32, Streptomyces thermoviolaceus OPC-250 chi25 (AB016843); 33, Streptomyces thermoviolaceus OPC-250 chi35 (AB016842); 34, Nocardiopsis prasina OPC-131(AB086832); 35, Nonomuraea spiralis; 36, Planobispora rosea fragment 1; 37, Planobispora rosea fragment 2; 38, Planomonospora parontospora subsp. antibiotica fragment 1; 39, Planomonospora parontospora subsp. antibiotica fragment 2.