Figure 8.

Osteogenic differentiation of human mesenchymal stem cells (hMSCs) cultured on plastic culture dish, dorsal shield block, and lamellar block. Cells were cultured for 5 days in the presence of an osteogenic stimulant (OS). Real-time polymerase chain reaction analysis for the expression of the osteoblast markers alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and collagen type I α 1 (ColIα1) showed a significant increase in hMSCs cultured on dorsal shield and lamellar blocks. Values are expressed as the mean ± SD. Significance was determined at *p < 0.05 when compared with non-OS-treated cells.