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Mung bean coat (MBC) extract reduced bacterial endotoxin-induced HMGB1 release. Murine macrophages were stimulated with LPS in the absence or presence of MBC extract at indicated concentrations. At 16 h after LPS stimulation, levels of HMGB1 in the culture medium or whole cell lysate were determined by Western blotting analysis. Western blots shown were representative results of three independent experiments. Means not sharing a common letter differ, P < 0.05.
