TABLE 5.
Kinetic parameters
| Method, environment, and organism | Generation time (h) | Oxidation rate (fmol of NH4+/h/cell) | Yield (106 cells/μmol) | Reference |
|---|---|---|---|---|
| Fluorescent-antibody staining, pure culture | ||||
| Nitrosospira | 20.1 | 4 | 8.0 | 1 |
| Nitrosolobus | 23 | 23 | 1.9 | |
| Acridine orange direct counting, pure culture, Nitrosospira spp. | 50 | 4.3 | 2.8 | 22 |
| 58 | 6.0 | 1.8 | ||
| 63 | 5.3 | 1.7 | ||
| 138 | 7.1 | 0.7 | ||
| Real-time PCR | ||||
| Microcosms, indigenous AOB, 1.5 mM NH4+ | 28 (0-3)a | 15.6 (0-1) | 5.6 (0-7) | This study |
| 1.0 (1-3) | ||||
| 0.8 (3-7) | ||||
| 0.4 (7-14) | ||||
| Microcosms, indigenous AOB, 7.5 mM NH4+ | 51.7 (0-7) | 8.5 (0-1) | 17.5 (0-7) | This study |
| 3.5 (1-3) | ||||
| 1.1 (3-7) | ||||
| 0.2 (7-14) | ||||
| 0.5 (14-28) | ||||
| Soil, indigenous AOB, field expt 1 | 372.7 (1-39) | 7.2 (3-7) | 1.7 (3-39) | This study |
| 2.3 (7-13) | ||||
| 0.5 (13-39) |
a
Values in parentheses are experiment durations in days.