Table 1. Processing and refinement statistics for the first and last thaumatin data set at each temperature.

All samples initially diffracted beyond the resolution limit of our experimental geometry (1.52 Å). As the samples became damaged the resolution dropped and scaling was truncated so that 〈I〉/〈σ(I)〉 = 2 in the highest resolution bin (of a total of ten bins). R _merge, intensity statistics, mosaicity and unit-cell parameters were determined with DENZO/SCALEPACK (Otwinowski & Minor, 1997 ▶). R values and atomic B factors were determined with BUSTER-TNT (Blanc et al., 2004 ▶). Data sets at intermediate doses exhibited statistics that interpolated between those of the first and last data sets within error.

	25 K		100 K		180 K		240 K		300 K
Data set	First	Last	First	Last	First	Last	First	Last	First	Last
PDB code	4ek0	4eka	4ekb	4ekh	4eko	4ekt	4el2	4el3	4el7	4ela
Resolution (Å)	1.52	1.55	1.52	1.75	1.52	1.75	1.52	1.95	1.52	2.00
Unit-cell parameters (Å)
a = b	57.130	57.350	57.390	57.510	57.770	57.900	58.270	58.320	58.370	58.330
c	150.30	150.78	149.74	149.90	149.94	150.07	150.72	150.75	151.27	151.30
〈I〉	3092.1	2074.8	1909.7	1232.8	657.4	409.0	373.2	131.6	254.7	106.6
〈I〉/〈σ(I)〉	37.66	31.67	33.68	27.09	26.29	21.87	22.89	13.70	20.37	12.84
Reflections	38888	37321	39378	26246	39290	26034	38012	18682	35453	17277
R merge (%)	8.4	8.8	8.5	11	7.5	8.8	7.8	9.9	8.2	13
Wilson B (Å2)	15.39	22.39	16.67	24.93	16.00	23.20	18.44	25.29	18.34	26.93
Mean B (Å2)	19.16	26.04	20.22	27.53	19.17	26.39	23.28	29.94	24.06	31.95
R/R free (%)	18.4/20.1	19.1/20.0	17.5/19.3	18.1/21.1	17.0/18.8	17.5/20.1	16.2/19.2	16.2/20.2	16.3/18.0	16.3/19.2
Mosaicity (°)	0.232	0.442	0.194	0.433	0.190	0.269	0.057	0.147	0.041	0.163