Figure 6.

Sepharose CL-6B-100 profiles of uronic acids released from mature-green cv Ailsa Craig cell walls by PG2 at 25 or 100 mm KCl over the pH range from 4.0 to 6.0. Cell walls (5 mg) in 2 mL of 20 mm KOAc at the indicated pH and KCl concentrations were incubated with the enzyme for 2 h at 34°C. Vo, Void volume; Vt, total volume.