FIG. 2.
Evaluation of the cell concentration and DNA purification assay with five different C. jejuni strains. The bacteria were spiked directly in bacterial binding and washing buffer (Genpoint), with subsequent analysis by 5′-nuclease PCR. A 10-fold dilution series, from approximately 102 to 106 CFU/ml, is shown. The ΔCT value were obtained by subtracting the CT value for each dilution from the CT value obtained for the log signal for approximately 6 log10 CFU/ml. The following C. jejuni strains were analyzed: •, LCD 8617; ○, ATCC 43438; ×, ATCC 43436; *, ATCC 43439; and +, ATCC 43442. A linear regression curve is shown.