FIG. 4.

PCA of the comparison of methods. The data presented in Table 2 were subjected to PCA (see Materials and Methods for details). (A) Score diagram for the methods tested. (B) Loading for the different parameters tested. The two PCs (PC1 and -2) explain 50 and 38% of the variance in the data, respectively. AMPEFF, amplification efficiency; REGRESSCO, squared regression coefficient; DETECTLIM, detection limit; time, performance time; CENTRIFUG, centrifugation; PRIMDIMER, primer dimer; BUGSB, Bugs'n Beads; DNADIR, DNA DIRECT.