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Amount of fecal or cecal content tolerated in a 50-μl reaction without detectable PCR inhibition
| Matrix | Purifi- cationa | Amt of matrix (mg) in presence of facilitator
|
|||
|---|---|---|---|---|---|
| No facilitator | 0.4% BSA | 4% PEG | 0.4% BSA and 4% PEG | ||
| Feces | Purified | 2.5 | 11 | 5 | 10 |
| Cecum | Purified | 0.1 | 1 | <0.2 | 1 |
| Feces | None | <0.5 | 0.5 | Not tested | Not tested |
| Cecum | None | <0.1 | <0.1 | Not tested | Not tested |
Purified material was purified with the Bugs'n Beads kit from Genpoint.
