TABLE 2.
Comparison of sample preparation methods
| Method | Amplification efficiency | Square regression coefficient (S2) (%) | Detection limita | Performance time | Centrifugation | Primer dimersb |
|---|---|---|---|---|---|---|
| Bugs'n Beads | 0.9 | 99.6 | >2-<20 | 30 min | No | No |
| DNeasy | 0.9 | 99.9 | >2-<20 | 4 h | Yes | Yes |
| Prepman | 1.0 | 99.8 | >2-<20 | 30 min | Yes | Yes |
| DNA DIRECT | 0.7 | 99.2 | >20-<200 | 45 min | Yes | Yes |
a
The detection limit is between the sample with the lowest CFU giving a signal and the next sample. The values are given relative to the CFU in the original sample.
b
The level of primer dimer formation was determined by qualitative PCR.