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Relative band intensities of Western immunoblot detection of ChrR in P. putida KT2440 grown in LB medium with (▪) or without (•) 400 μM Cr(VI). The 8-h (darkest) band intensity, seen in cultures containing chromate, was taken as 100%. The inset shows Western bands for the challenged and unchallenged samples. The numbers indicate the collection time points in hours; 26 μg of total protein was loaded in each lane. Qualitatively similar results were seen in two independent measurements.
