Sequence analysis of AgCHS genes. A) Gene structures of AgCHS1 and AgCHS2. AgCHS1 has two splice variants with alternative splicing exons (6A and 6B). B) Phylogenetic tree of insect chitin synthases. C) Phylogenetic tree of the alternative exons of insect CHS1. Ag, Anopheles gambiae; Ae, Aedes aegypti; Aq, A. quadrimaculatus; Dm, Drosophila melanogaster; Lc, Lucilia cuprina; Lm, Locusta migratoria; Ms, Manduca sexta; Px, Plutella xylostella; Tc, Tribolium castaneum; Se, Spodoptera exiqua; Sf, Spodoptera frugi; Eo, Ectropis obliqua; Ce, Caenorhabditis elegans. GenBank Accession numbers are listed in Table 2. Phylogenetic tree was generated using the software MEGA 5 after alignment by using ClustalW method.