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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2012 Jun 7;42(9):674–682. doi: 10.1016/j.ibmb.2012.05.005

Fig. 9.

Fig. 9

Immunohistochemistry of anti-AgCHS1 (A, B) and anti-AgCHS2 serum (C, D) in the pupal stage of An. gambiae. Paraffin-embedded thin sections of the 12- to 24-hour-old pupae were immunostained with primary antibodies and visualized by the reaction with Alexa 488-conjugated goat anti-mouse IgG. The epidermal cells in the adult cuticle (A) and the eyes (B) were immunoreactive (arrows indicate positive staining, arrow heads indicate negative staining). The abdominal inter-segmental region of the pupal cuticle (C) and the eyes (D) were immunoreactive (arrows indicate positive staining).