TABLE 2.
Primers used in this study
| Oligonucleotide sequencea | Amino acidb | Primer name |
|---|---|---|
| CAC GCT TTC CCG AGC GCA GCT | Phe | None (WT) |
| CAC GCT CTG CCG AGC GCA GCT | Leu | EcfabH(F87L) |
| CAC GCT TCC CCG AGC GCA GCT | Ser | EcfabH(F87S) |
| CAC GCT TAC CCG AGC GCA GCT | Tyr | EcfabH(F87Y) |
| CAC GCT TGC CCG AGC GCA GCT | Cys | EcfabH(F87C) |
| CAC GCT TGG CCG AGC GCA GCT | Trp | EcfabH(F87W) |
| CAC GCT CCG CCG AGC GCA GCT | Pro | EcfabH(F87P) |
| CAC GCT CAC CCG AGC GCA GCT | His | EcfabH(F87H) |
| CAC GCT CAG CCG AGC GCA GCT | Gln | EcfabH(F87Q) |
| CAC GCT CGT CCG AGC GCA GCT | Arg | EcfabH(F87R) |
| CAC GCT ATC CCG AGC GCA GCT | Ile | EcfabH(F87I) |
| CAC GCT ATG CCG AGC GCA GCT | Met | EcfabH(F87M) |
| CAC GCT ACC CCG AGC GCA GCT | Thr | EcfabH(F87T) |
| CAC GCT AAC CCG AGC GCA GCT | Asn | EcfabH(F87N) |
| CAC GCT AAA CCG AGC GCA GCT | Lys | EcfabH(F87K) |
| CAC GCT GTG CCG AGC GCA GCT | Val | EcfabH(F87V) |
| CAC GCT GCG CCG AGC GCA GCT | Ala | EcfabH(F87A) |
| CAC GCT GAT CCG AGC GCA GCT | Asp | EcfabH(F87D) |
| CAC GCT GAA CCG AGC GCA GCT | Glu | EcfabH(F87E) |
| CAC GCT GGC CCG AGC GCA GCT | Gly | EcfabH(F87G) |
| CGT AGC AGA AGT CGT TGC CAC | Reverse | EcfabH(rev) |
| TGC TTC TGG CGT CAG GCA GCC ATC G | —c | pTrc99F(38) |
| CGA TGG CTG CCT GAC GCC AGA AGC A | — | pTrc99R(62) |
| CAC GAG GGA GCT TCC AGG GGG A | — | pTrc99F(2350) |
| TCA CGT AGC GAT AGC GGA GT | — | pTrc99R(2777) |
| ACT CCG CTA TCG CTA CGT GA | — | pTrc99F(2767) |
| CAA ATC GGC GTT TGT CCG CAC TTG | — | L22 REV |
| ATG GTG GTT AAC GGC GGG ATA TAA C | — | pTrc99R(3920) |
| CAG ACT GGA GGT GGC AAC GCC AAT CA | — | pTrc99R(3269) |
a
Bold face lettering indicates the changed codon. Codon changes were based on E. coli codon usage frequency.
b
Amino acid coded for at position 87 of the E. coli FabH protein.
c
—, sequencing primers for pTrc99A.